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Image Search Results
Journal: EMBO Reports
Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer
doi: 10.15252/embr.202256524
Figure Lengend Snippet: A Experimental setting of bone marrow differentiation. B Representative dot plots of CD11b and Ly6G staining in bone marrow cells cultured in the presence of Epi cells, PeSCs or both Epi cells, and PeSCs. C Quantification of the percentage of Ly6G + cells among CD11b + cells (cumulative from three independent experiments). The bars are representing the mean ± SD. D Experimental setting for in vivo injection. FACS analysis of the percentages of Ly6G + CD45 + cells (each dot represents one mouse per group). E, F Representative immunofluorescence staining of coimplanted Epi cells + PeSCs for Ly6G, mCherry, Nanog, and DAPI. Scale bar, 50 mm. G–M In vivo depletion of Ly6G + MDSCs diminishes tumor growth. (G) Experimental setting. (H) Tumor weight. FACS analysis of the percentage of CD45 + cells (I), and Ly6G + MDSCs (J) derived from the tumor. (K) Representative dot plots of Ly6G‐ and F4/80‐stained CD45 + cells. FACS analysis of the percentage of F4/80 + cells. (L) Representative dot plots of CD45‐ and CD11c‐stained cells. FACS analysis of the percentage of CD11c + cells among CD45 + cells. (M) FACS analysis of GrzB + , TNFα + , and IFNγ + cells among CD45 + cells. Eight mice in each group, five for FACS analysis and three for histological analysis. Each dot represents one mouse. Data information: * P < 0.05, ** P < 0.01, and *** P < 0.001. The P ‐values were calculated using Student's t ‐test. Source data are available online for this figure.
Article Snippet:
Techniques: Staining, Cell Culture, In Vivo, Injection, Immunofluorescence, Derivative Assay
Journal: iScience
Article Title: Acquisition of neural fate by combination of BMP blockade and chromatin modification
doi: 10.1016/j.isci.2023.107887
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Knock-Out, Isolation, Purification, Imaging, Western Blot, Software, CRISPR